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Zymo Research 16s rrna gene v3 v4 primer pairs
AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
16s Rrna Gene V3 V4 Primer Pairs, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Zymo Research 16s rrna v3 v4 gene
AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
16s Rrna V3 V4 Gene, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
Data 16s Rrna Gene Sequencing Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Zymo Research 16s rrna v3 v5 gene region
AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
16s Rrna V3 V5 Gene Region, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
16s Rrna Gene V3 V4 Hypervariable Region, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RTLGenomics 16s rrna gene v3-v4 regions
AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
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Illumina Inc high-quality bacterial 16s rrna gene v3-v4 sequences
AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
High Quality Bacterial 16s Rrna Gene V3 V4 Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Zymo Research 2023 16s rrna gene variable region v3 v4 obese adults fecal
AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
2023 16s Rrna Gene Variable Region V3 V4 Obese Adults Fecal, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Illumina Inc 16s rrna gene v3–v4 region primers
AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS <t>16S</t> <t>V3–V4</t> mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.
16s Rrna Gene V3–V4 Region Primers, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS 16S V3–V4 mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.

Journal: Microbiology Resource Announcements

Article Title: AmpliFuse: an amplicon simulation tool with enhanced chimera generation for Illumina platforms

doi: 10.1128/mra.01439-25

Figure Lengend Snippet: AmpliFuse workflow and representative chimera formations with alignment analyses. ( A ) Overview of the AmpliFuse workflow. ( B and C ) Schematic of representative chimera formations using datasets derived from ZymoBIOMICS 16S V3–V4 mock community ( B ) and the AMR panel ( C ). The expected breakpoint (dashed line) lies within a microhomology (MH) highlighted in yellow. ( D and E ) Example alignments of two parent amplicons to a representative chimeric amplicon generated from the ZymoBIOMICS ( D ) and AMR panel ( E ) datasets, respectively. Under the default settings, chimeric amplicons account for approximately 15.4 and 4.9% of all amplicons generated using the ZymoBIOMICS and the AMR panel datasets, respectively.

Article Snippet: AmpliFuse was demonstrated utilizing two publicly available datasets, namely, ZymoBIOMICS Microbial Community ( https://zymoresearch.eu/collections/zymobiomics-microbial-community-standards/products/zymobiomics-microbial-community-standard ) using 16S rRNA gene V3–V4 primer pairs (341F and 805R) ( ) and the Illumina AmpliSeq AMR Research Panel (the AMR panel) ( https://www.illumina.com/products/by-brand/ampliseq/community-panels/antimicrobial-resistance.html ).

Techniques: Derivative Assay, Amplification, Generated